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Cell Line:	NCI-H1299
Cell Type:	epithelial lung cell
Description:	Human lung carcinoma
other names:	H1299, CRL-5803
application:	pDNA
Recommendation:	ScreenFect A

Recommendation based on in-house testing or customer feedback.

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Recommendation: ScreenFect®A

Transfection conditions*

Format:	96-well	24-well	6-well
ScreenFect volume:	0,375 µl	2,1 µl	6 µl
Amount of nucleic acid:	75 ng	300 ng	1000 ng
DNA:reagent ratio (µg:µl):	1:5	1:7	1:6
Cell number (cells/well)	20.000-25.000	-	-
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual

Transfection efficiency:	77 %	-	-
Viability:	92%	-	-

ScreenFect Enhancer addition leads to the following results:
Transfection efficiency increase:	7%	-	-

Note: The Two-Step method leads to slightly decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:4, Transfection performance can be increased by 20% using ScreenFect Enhancer for 96 well format.

Alternative product: ScreenFect®A plus

Format:	96-well	24-well	6-well
ScreenFect volume:	0,225µl	1,2 µl	4 µl
Amount of nucleic acid:	75 ng	300 ng	1000 ng
DNA:reagent ratio (µg:µl):	1:3	1:4	1:4
Cell number (cells/well)	20.000-25.000	-	-
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual

Transfection efficiency:	76 %	-	-
Viability:	90%	-	-

ScreenFect Enhacer addition leads to the following results:
Transefction efficiency increase:	6%	-	-

Note: The Two-Step method leads to slightly decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:2, Transfection performance can be increased by 11% using ScreenFect Enhancer for 96 well format.